of Phytocompounds of C.guianensis.
chromatography and Mass spectroscopy have a various field of applications. The primary
area of use is in the separation and analysis of multi- component mixtures such
as essential oils, hydrocarbons and solvents 12.Gas Chromatography Mass
Spectroscopy, a hyphenated system which is a very compatible technique and the
most commonly used technique for the identification and quantification of
phytocompounds from the herbal medicinal plant origin The unknown phytocomponents in a complex mixture can be determined by the
interpretations by matching the spectra with known reference spectra 13.
GC-MS chromatogram of the ethyl acetate extract of Couroupita guianensis (Fig1) shows 17 peaks and has been identified after comparison of the mass
spectra with NIST_MSMS, REPLIB and MAINLIB, indicating the presence of 11
phytocompenents 14. The secondary metabolites with their retention time, Molecular
formula, Molecular weight and concentration
peak area percentage were presented in Table 1. GC-MS analysis of ethyl
acetate extract revealed the presence of 11 different compounds namely
N-(-)-jasmonoyl-(s)- , Isopropyl amine, Tetracosane, Nonadecane, Octacosane,
Squalene, Ergosta-7,22-dien-3-, 4,4,6a,6b,8a,11,11, Alpha-amyrin,
Lup-20(29)-en-3-one, Lupeol. The retention time ,molecular weight and the
relative percentages of the compounds present in aerial parts of Couroupita guianensis were recorded in Figure
1 and table 1.
GC-MS spectrum confirmed the presence of 11 major components with the retention
time 2.053, 2.12. 31.98, 33.07, 34.13, 35.17, 36.18, 36.18, 36.80, 37.17, 38.16,39.15,40.16,
44.15, 45.03, 45.33, 46.34 and 46.64 respectively (Table 1). The name of the
compound, molecular weight of active principles, molecular formula and
structure of the component of the plant material t material were determined
15. N-(-)-jasmonoyl-(s)- is endogenous bioactive jasmonateand it has the
hormone triggered activation of jasmonate signaling pathways and structurally
similar to coronatine 16.
Isopropylamine (2- Aminopropane) is a substances which is used as a food flavouring agent.
The pure chemical is corrosive to the eyes, skin, and respiratory tract.
Contact with the liquid or vapor can cause severe burns of the eyes and skin;
can cause blurred vision or seeing halos around lights, with loss of vision and
scarring. Corrosive on ingestion. Swallowing the liquid may cause chemical
pneumonitis. Higher exposures can cause pulmonary edema and this can cause death 17. Figure 2 shows the HPLC analysis of ethyl acetate extract
of aerial parts of couroupita guianensis
was carried out with the mobile phase methanol: water in the ratio 95:5 gave a
total of 25 peaks at retention time 2.171, 2.871, 3.014, 3.637, 4.048, 4.455,
4.580, 4.741, 5.417, 5.896, 6.676, 7.311, 8.563, 9.343, 10.574, 10.826, 12.430,
13.069, 14.607, 15.231, 16.504, 16.957, 19.473, 25.670, 30.669 (Figure : 2).
The highest peak was seen at the retention time 3.014 minute 17. Figure 3 shows the antibacterial activity of the control drug
penicillin and ethyl acetate extract of Couropita guianensis on selected human
bacterial pathogens. The extract of the
plant shows more significant effects on the pathogens as compared to penicillin
in particularly the ethyl acetate extract of Couropita guianensis shows the
considerable antibacterial efficacy on P.aeroginosa, B.cereus and S.flexneri as
compared to S.typhi and E.coli. Figure 4 shows the minimum fungicidal
concentration of the control drug Penicillin and ethyl acetate extract of the
medicinal plant Couropita guianensis against selected human fungal pathogens.
Ethyl acetate extract of C.guianensis shows significant efficacy against fungal
pathogens especially Mucor sp, Candida albicans and A.fumigatus as compared to
A.niger and A.flavus this indicates that the plant extract possesses the strong
antifungal activity. Plant extracts or plant based compounds or phytocompounds
are likely to provide a highly valuable source of new medicinal agents The
present study emphasizes that the antibacterial and antifungal activity of
couropita guianensis on selected human pathogens due to the presence of various
phytochemical compounds and their antioxidant potential.
identification of different biologically active compounds in the ethyl acetate
extract of a medicinal plant Couropita
guianensis and their role in antimicrobial efficacy on selected human
pathogens warrants further biological and pharmacological studies.