Data obtained from microbial analysis of samples collected from the different bathrooms of public and private residences of university students in this study showed that different bacterial organisms were contaminating the walls. . The following agars were used for the study; MacConkey agar, Nutrient Agar and Eosin Methylene Blue Agar (EMB), Manitol Salt Agar (MSA). Organisms isolated were distinguished morphologically and more distinguished using some Biochemical tests including Catalase, Methyl Red, Voges Proskauer, Oxidase Test, Citrate Utilization Test, Triple Sugar Iron Test and Urease Activity Tests.Colony formation units per gram of samples collected in Table 1 showed that the walls of public bathrooms were more contaminated than those of private bathrooms and Incidence and percentage occurrence of bacteria isolates from private and public bathrooms.
Results obtained from biochemical tests to further confirm identification of organisms isolated from samples showed different gram-positive and gram-negative bacteria species present (Tables 2 and 3) which belong to the genus Staphylococcus, Escherichia, and Enterococcus etc. were some of the organisms identified. In (Table 2) the isolated organisms are Enterobacter, Staphylococcus.auerus, Shigella and Escherichia.coli. The percentage of Enterobacter, Staphylococcus.auerus, Shigella and Escherichia.coli contamination in the private bathrooms was 30%, 40%, 10% and 20%. But in (Table 3) the isolated organisms are Salmonella, Staphylococcus aureus, Shigella, Enterococcus, Klebsiella, Proteus, Serratia, Pseudomonas and Escherichia coli. The percentage of Salmonella, Staphylococcus aureus, Shigella, Enterococcus, Klebsiella, Proteus, Serratia, Pseudomonas and Escherichia coli contamination in public bathrooms was 21%, 26.3%, 5.2%, 10.5%, 21%, 5.2%, 5.2%, 5.2%. Both table 1 and 2 shows the Public bathrooms are contain more pathogenic organisms as compare to the private bathrooms.
The ratio of bathroom users the number of the bathrooms is also very important because results from the study indicated that swabs from walls of public bathrooms had more microbial load than those from walls of private bathrooms and organisms such as Serretia, Klebsiella, pseudomonas, Proteus were isolated from public bathrooms were not present in the private bathrooms.