Aseptic technique is a method used to keep the work and worker
free of bacterial contamination. Louis Pasteur was a
French scientist who lived in the 19th century. He discovered the first vaccine
for rabies, but is more famous for the aseptic technique. Bacteria are found everywhere,
and some are good for us while others are harmful due to they cause diseases. Microbiologists, scientists, and doctors use aseptic technique to
stop contamination by microorganisms in surgery rooms, clinics, laboratories
and many more places. It is a combination of laboratory methods which are used to keep microorganisms
from growing in an area. It can also prevent the spread of a microorganism from
one area to another. It is used for a
variety of procedures such as transferring cultures, isolation of pure
cultures, helping with a baby’s birth by vaginal delivery performing various surgical
techniques and more.
starting an experiment that needs the aseptic technique you will need disposable
sterile petri dishes to hold bacterial cultures, sterile culture tubes,
disposable pipettes, bunsen burner, inoculation loop, and a sterile cabinet. The
aseptic technique procedure is prepare and clean
the work area before beginning and set up materials. One will then flame the
inoculating loop before making a transfer of bacteria from one container to
another. Never lay an inoculating loop on the bench top if you are not sure it
has been flamed first. Next, flame the opening of glass containers before
removing bacteria from them and do not lay the cap of containers of bacteria on
the bench top while bacteria are removed from or transferred to the container.
The cap should remain under your control throughout the transfer. Make sure one
works quickly and efficiently to minimize the time the culture is exposed to
the environment. Once you are done flame the opening of the glass container after
transferring bacteria to another container. One should close everything securely
and put materials back properly. Finally, clean work area again to protect other
from possible contaminations.
The importance of the aseptic technique is to prevent
contamination on the one doing the experiment, others, and the actual experiment.
The aseptic technique is beneficial, because it prevents the smallest cut on
your body during lab or people undergoing surgery from getting infected. If the
procedures are not done correctly or not used at all this could cause time
wasted, bacterial mutation, infections, sickness, and even death.